Journal: Human Cell

Article Title: Establishment of Coala: a novel 3D and 2D cancer cell line derived from colorectal cancer liver metastasis

doi: 10.1007/s13577-025-01256-1

Figure Lengend Snippet: Characterization of newly-derived Coala cancer cell line. Cell line was derived from human colorectal cancer liver metastasis ( a ), forms sharply-edged, fast growing colonies in 2D ( b – d ), when embedded into Matrigel matrix, the cell line form spherically-shaped tumorspheres ( e , f ). Image g shows the phenotype of tumorspheres, when Coala cells are cultured in ultra-low attachment plates. Scale bars represent 50 µm ( b – d and k , l ) and 500 µm ( e – g ). Multicolor (M-FISH) analysis of Coala cells showing the abnormal karyotype ( h ). Negative PCR test (detection of multiple mycoplasma species) in Coala cell culture ( i ). Antibody array chip analysis shows the expression of Snail, FoxA2, E-Cadherin and Pdx-1 in Coala cell lysate. Cultured Coala cells show positivity for Cytokeratin 20 (DAPI was used for nuclei staining), scale bar represents 50 µm. Growth curve for Coala cells ( m ) and effect of 5-Fluorouracil treatment ( n ). Image ( o ) showing a tumor formed in athymic mouse 3 weeks after subcutaneous grafting of Coala cells. Images ( p ) and ( r ) show flow cytometry analysis of Lgr5 marker in early passage (P3) of Coala cells. Cells from passage No.21 were used in experiments

Article Snippet: Expression of 19 transcription factors was detected with Stem Cell Antibody Array (RD Systems, UK).

Techniques: Derivative Assay, Cell Culture, Ab Array, Expressing, Staining, Flow Cytometry, Marker